Expression of the MMP-1 in human pancreatic carcinoma: relationship with prognostic factor

Mod Pathol. 1999 Jul;12(7):669-74.


Matrix metalloproteinases (MMPs) have been implicated in the invasion and metastasis of tumor cells. To elucidate the involvement of MMP-1 in human pancreatic ductal adenocarcinoma, we performed immunohistochemical analysis on tissues from 2 fetal pancreases, 5 normal pancreases, 6 cases of chronic pancreatitis, and 46 pancreatic ductal adenocarcinomas. In addition, among the pancreatic carcinomas, we compared MMP-1 expression in relation to the degree of differentiation, lymph node metastasis, and depth of invasion of the carcinoma. MMP-1 was expressed faintly in fetal and normal pancreatic tissues. Among the 46 pancreatic carcinomas, 33 (72%) showed positive staining for the MMP-1 protein. There was no difference in the degree of differentiation. In situ hybridization confirmed the presence of MMP-1 mRNA in the pancreatic carcinomas. Expression of MMP-1 mRNA was also detected in two human pancreatic carcinoma cell lines and three pancreatic carcinoma tissues by the reverse transcription polymerase chain reaction method. MMP-1 was expressed in the carcinoma cells themselves and in stromal fibroblasts. Patients with MMP-1 positivity in the primary site had a significantly poorer prognosis than patients who were MMP-1 negative (P < .05). MMP-1 expression, however, had no relation to the presence of lymph node metastasis, tumor size, or tumor-node-metastasis stage in pancreatic carcinomas. These findings suggest that MMP-1 expression is related to the carcinogenesis and prognosis of human pancreatic ductal adenocarcinoma.

MeSH terms

  • Adenocarcinoma / enzymology
  • Adenocarcinoma / genetics*
  • Adenocarcinoma / pathology
  • Aged
  • Aged, 80 and over
  • Collagenases / analysis
  • Collagenases / genetics*
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization
  • Male
  • Matrix Metalloproteinase 1
  • Middle Aged
  • Pancreas / enzymology
  • Pancreatic Ducts / pathology
  • Pancreatic Neoplasms / enzymology
  • Pancreatic Neoplasms / genetics*
  • Pancreatic Neoplasms / pathology
  • Prognosis
  • RNA / genetics
  • Reverse Transcriptase Polymerase Chain Reaction


  • RNA
  • Collagenases
  • Matrix Metalloproteinase 1