The functional intronless S-adenosylmethionine decarboxylase gene of the mouse (Amd-2) is linked to the ornithine decarboxylase gene (Odc) on chromosome 12 and is present in distantly related species of the genus Mus

Mamm Genome. 1999 Aug;10(8):784-8. doi: 10.1007/s003359901092.

Abstract

S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in the biosynthesis of polyamines. We have previously identified a mouse AdoMetDC gene that exhibits the hallmarks of a retroposon; that is, it has no introns, is flanked by direct repeats, and has a poly(dA) tract at its 3'-end. This gene, termed Amd-2, is not a processed pseudogene; rather, it is transcribed in a variety of mouse tissues and encodes a functional enzyme. In the current report, we present the sequence of a 6.7-kb genomic segment of the Amd-2 locus. Several sequences of interest, including an intercisternal A particle (IAP) element, a transposon-related sequence, and several expressed sequence tags (ESTs), were found within or near Amd-2. We also show, through analysis of an interspecific backcross, that Amd-2 is located on Chr 12, tightly linked to the gene (Odc) that encodes ornithine decarboxylase, another key enzyme in polyamine synthesis. Finally, we show that Amd-2 is present among several divergent species of the genus Mus. Thus, the integration event that generated Amd-2 may have occurred early during Mus evolution.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosylmethionine Decarboxylase / genetics*
  • Animals
  • Biological Evolution
  • Chromosome Mapping
  • Crosses, Genetic
  • DNA / genetics
  • Genetic Linkage
  • Introns
  • Mice / genetics*
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Muridae / genetics*
  • Ornithine Decarboxylase / genetics*
  • Phylogeny
  • Species Specificity

Substances

  • DNA
  • Ornithine Decarboxylase
  • Adenosylmethionine Decarboxylase

Associated data

  • GENBANK/AF052604