Gas chromatographic determination of galactose in milk. Example of a switching valve used for the protection of the capillary column

J Chromatogr A. 1999 Jun 25;847(1-2):47-51. doi: 10.1016/s0021-9673(99)00032-1.

Abstract

Galactose, a marker of heat treatment, has been analysed in milk as pentafluorobenzyloxime acetate by gas chromatography with flame ionization detection using a simple switching valve system. The procedure did not entail any prederivatization clean-up for lactose elimination from the sample. In a short pre-column, reagent and lactose derivative excess were separated and the galactose and internal standard derivatives were transferred to the analytical column by a four-port valve. Thus, the analytical column was protected from overloading, so avoiding rapid deterioration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, Gas / instrumentation
  • Chromatography, Gas / methods*
  • Galactose / analysis*
  • Milk / chemistry*
  • Reference Standards
  • Reproducibility of Results

Substances

  • Galactose