Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy

Gene Ther. 1999 Feb;6(2):198-208. doi: 10.1038/


We describe the construction of retroviral plasmid vectors in which two genes are linked by a minimum of 96 nucleotides encoding the 2A sequence from the picornavirus foot-and-mouth disease virus (FMDV). Transcription and translation gives rise to a bicistronic mRNA and two independent protein products. The system offers advantages to other alternative ways to create polycistronic mRNAs and can be used in gene therapy delivery vectors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Acetyltransferases / genetics
  • Animals
  • Antiviral Agents / pharmacology
  • Aphthovirus / genetics*
  • Blotting, Western
  • Chimera
  • Ganciclovir / pharmacology
  • Gene Expression
  • Genetic Therapy / methods*
  • Genetic Vectors*
  • Genome, Viral
  • Humans
  • Mice
  • Plasmids
  • Thymidine Kinase / genetics
  • Tumor Cells, Cultured


  • Antiviral Agents
  • Acetyltransferases
  • puromycin N-acetyltransferase
  • Thymidine Kinase
  • Ganciclovir