Bullous pemphigoid IgG2 and IgG4 antibodies have a capability to inhibit a transient increase of intracellular Ca2+ induced by bullous pemphigoid IgG1 antibody

Autoimmunity. 1999;30(2):85-91. doi: 10.3109/08916939908994765.

Abstract

To elucidate the role of Bullous pemphigoid (BP) IgG subclasses in the transmembrane signal transduction of keratinocytes, we examined whether BP IgG2 or IgG4 inhibits the intracellular Ca2+ concentration ([Ca2+]i) increase induced by BP IgG1. IgG2 from one of five BP patients inhibited the increase of [Ca2+]i in human squamous cell carcinoma cell line; DJM-1 cells induced by BP IgG1. IgG4 from two of four BP patients inhibited the increase of [Ca2+]i induced by BP IgG1. In addition, a two fold quantity of IgG2 inhibited the increase of [Ca2+]i induced by BP IgG1. A two fold quantity of IgG4 from two of five BP patients inhibited the increase of [Ca2+]i induced by BP IgG1. These results indicate that BP IgG2 and IgG4 have a capability to inhibit the increase of [Ca2+]i induced by BP IgG1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoantibodies / immunology*
  • Autoantibodies / pharmacology*
  • Autoantigens / immunology
  • Calcium Signaling / drug effects*
  • Calcium Signaling / immunology
  • Carcinoma, Squamous Cell / pathology
  • Carrier Proteins*
  • Collagen Type XVII
  • Collagen*
  • Cytoskeletal Proteins*
  • Dystonin
  • Humans
  • Immunoglobulin G / immunology*
  • Immunoglobulin G / pharmacology*
  • Keratinocytes / metabolism
  • Nerve Tissue Proteins*
  • Non-Fibrillar Collagens*
  • Pemphigoid, Bullous / immunology*
  • Tumor Cells, Cultured

Substances

  • Autoantibodies
  • Autoantigens
  • Carrier Proteins
  • Collagen
  • Cytoskeletal Proteins
  • Dystonin
  • Immunoglobulin G
  • Nerve Tissue Proteins
  • Non-Fibrillar Collagens
  • Collagen Type XVII
  • DST protein, human