The sae locus of Staphylococcus aureus encodes a two-component regulatory system

FEMS Microbiol Lett. 1999 Aug 1;177(1):15-22. doi: 10.1111/j.1574-6968.1999.tb13707.x.


Sae is a regulatory locus that activates the production of several exoproteins in Staphylococcus aureus. A 3.4-kb fragment of a S. aureus genomic library, screened with a probe adjacent to the transposon insertion of a sae::Tn551 mutant, was cloned into a bifunctional vector. This fragment was shown to carry the sae locus by restoration of exoprotein production in sae mutants. The sae locus was mapped to the SmaI-D fragment of the staphylococcal chromosome by pulse-field electrophoresis. Sequence analysis of the cloned fragment revealed the presence of two genes, designated saeR and saeS, encoding a response regulator and a histidine protein kinase, respectively, with high homology to other bacterial two-component regulatory systems.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Bacterial / genetics
  • Cloning, Molecular
  • Conserved Sequence
  • Gene Expression Regulation, Bacterial*
  • Genes, Regulator*
  • Genomic Library
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Protein Kinases / biosynthesis
  • Protein Kinases / chemistry
  • Protein Kinases / genetics*
  • Restriction Mapping
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / metabolism


  • Bacterial Proteins
  • Protein Kinases
  • SaeS protein, Staphylococcus aureus

Associated data

  • GENBANK/AF129010