Tumour-associated E-cadherin mutations alter cellular morphology, decrease cellular adhesion and increase cellular motility

Oncogene. 1999 Jul 29;18(30):4301-12. doi: 10.1038/sj.onc.1202790.


A major function of the cell-to-cell adhesion molecule E-cadherin is the maintenance of cell adhesion and tissue integrity. E-cadherin deficiency in tumours leads to changes in cell morphology and motility, so that E-cadherin is considered to be a suppressor of invasion. In this study we investigated the functional consequences of three tumour-associated gene mutations that affect the extracellular portion of E-cadherin: in-frame deletions of exons 8 or 9 and a point mutation in exon 8, as they were found in human gastric carcinomas. Human MDA-MB-435S breast carcinoma cells and mouse L fibroblasts were stably transfected with the wild-type and mutant cDNAs, and the resulting changes in localization of E-cadherin, cell morphology, strength of calcium-dependent aggregation as well as cell motility and actin cytoskeleton organization were studied. We found that cells transfected with wild-type E-cadherin showed an epitheloid morphology, while all cell lines expressing mutant E-cadherin exhibited more irregular cell shapes. Cells expressing E-cadherin mutated in exon 8 showed the most scattered appearance, whereas cells with deletion of exon 9 had an intermediate state. Mutant E-cadherins were localized to the lateral regions of cell-to-cell contact sites. Additionally, both exon 8-mutated E-cadherins showed apical and perinuclear localization, and actin filaments were drastically reduced. MDA-MB-435S cells with initial calcium-dependent cell aggregation exhibited decreased aggregation and, remarkably, increased cell motility, when mutant E-cadherin was expressed. Therefore, we conclude that these E-cadherin mutations may not simply affect cell adhesion but may act in a trans-dominant-active manner, i.e. lead to increased cell motility. Our study suggests that E-cadherin mutations affecting exons 8 or 9 are the cause of multiple morphological and functional disorders and could induce the scattered morphology and the invasive behaviour of diffuse type-gastric carcinomas.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / ultrastructure*
  • Animals
  • Cadherins / genetics*
  • Cadherins / metabolism
  • Cell Adhesion*
  • Cell Movement*
  • Exons
  • Fibroblasts / cytology
  • Fluorescent Antibody Technique
  • Humans
  • Mammary Neoplasms, Animal / genetics
  • Mice
  • Microscopy, Confocal
  • Models, Genetic
  • Mutation*
  • Point Mutation
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / pathology
  • Transfection
  • Tumor Cells, Cultured
  • Wound Healing


  • Actins
  • Cadherins