Both CD4+ and CD8+ lymphocytes reduce the severity of tissue lesions in murine systemic cadidiasis, and CD4+ cells also demonstrate strain-specific immunopathological effects

Microbiology. 1999 Jul;145 ( Pt 7):1631-1640. doi: 10.1099/13500872-145-7-1631.

Abstract

The role of T lymphocytes in host responses to sublethal systemic infection with Candida albicans was evaluated by mAb depletion of CD4+ and CD8+ cells from BALB/c and CBA/CaH mice, which develop mild and severe tissue damage, respectively. Depletion of CD4+ lymphocytes from BALB/c mice markedly increased tissue damage, but did not alter the course of infection. In CBA/CaH mice, depletion of CD4+ cells abrogated tissue destruction in both brain and kidney at day 4 after infection, and significantly decreased fungal colonization in the brain. However, the severity of tissue lesions increased relative to controls from day 8 onwards. A small increase in tissue damage was evident in both mouse strains after depletion of CD8+ cells. There were no major differences between days 4 and 8 after infection in cDNA cytokine profiles of CD4+ lymphocytes from either BALB/c or CBA/CaH mice. After passive transfer into infected syngeneic recipients, spleen cells from infected CBA/CaH mice markedly increased tissue damage when compared to controls, and also caused a significant increase in fungal colonization in the brain. A similar transfer in BALB/c mice increased the number of inflammatory cells in and around the lesions, but had no effect on the fungal burden in brain and kidney. The data demonstrate that both CD4+ and CD8+ lymphocytes contribute to the reduction of tissue damage after systemic infection with C. albicans, and that the development and expression of CD4+ lymphocyte effector function is influenced by the genetic background of the mouse.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Animals
  • Brain / microbiology
  • Brain / pathology
  • CD4-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / immunology*
  • Candida albicans / immunology*
  • Candidiasis / immunology*
  • Candidiasis / microbiology
  • Candidiasis / pathology
  • Cytokines / biosynthesis
  • Cytokines / genetics
  • Female
  • Kidney / microbiology
  • Kidney / pathology
  • Lymphocyte Depletion
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred CBA
  • Species Specificity
  • Spleen / cytology
  • Spleen / immunology

Substances

  • Cytokines