Molecular cytogenetic studies of a serially transplanted primary prostatic carcinoma xenograft (CWR22) and four relapsed tumors

Prostate. 1999 Sep 15;41(1):7-11. doi: 10.1002/(sici)1097-0045(19990915)41:1<7::aid-pros2>3.0.co;2-1.

Abstract

Background: Established cell lines or xenografts from prostatic carcinoma have been infrequently studied cytogenetically. CWR22 and CWR22-R are xenografts that are unique in offering one strongly androgen-dependent and several relapsed strains of a human prostate cancer that can be investigated in the laboratory. We report on the cytogenetic characterization of the hormone-dependent CWR22, and the relapsed CWR22-R serially transplanted xenografts, in our laboratory.

Methods: We utilized a suspension harvest of the xenograft tissue to optimize our yield for metaphase chromosome studies and analyzed the hormone-dependent CWR22 and four relapsed CWR22-R xenografts. These studies were accomplished using standard G-banded analysis and fluorescence in situ hybridization (FISH). A variety of DNA probes including alpha-satellite DNA probes, and chromosomal libraries, were utilized for the FISH analysis.

Results: Utilizing both standard cytogenetic analysis and FISH studies we have more precisely defined the CWR22 xenograft: 49,XY,+i(1)(q10),-2, der(4)t(2;4)(p21;q33), +7,+8,+12[7]/50,XY,idem, +der(2)t(2;4)(p21;q33)del(2)(q13q33)[13]. Four relapsed xenografts, CWR22R-2152, CWR22R-2524, CWR22R-2274, and CWR22R-2272 were also studied. Each of these lines demonstrated a different karyotype.

Conclusions: The CWR22 karyotype offers the simplest reported karyotype for a prostate cancer tissue culture cell line or xenograft; this makes CWR22 an attractive candidate for studies of genetic changes associated with the relapse of prostate cancer treated with androgen withdrawal. Four separate, serially transplanted, relapsed CWR22-R xenografts were detected, each with a separate karyotype.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Androgens / physiology
  • Animals
  • Chromosome Aberrations / genetics
  • Chromosome Banding
  • Chromosomes, Human / genetics
  • Humans
  • In Situ Hybridization, Fluorescence
  • Karyotyping
  • Male
  • Mice
  • Mosaicism / genetics
  • Neoplasm Recurrence, Local
  • Neoplasm Transplantation*
  • Orchiectomy
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / pathology
  • Transplantation, Heterologous*
  • Tumor Cells, Cultured

Substances

  • Androgens