Three new types of Apaf-1 in mammalian cells

Biochem Biophys Res Commun. 1999 Aug 11;261(3):746-9. doi: 10.1006/bbrc.1999.1124.

Abstract

Apaf-1, the human C. elegans Ced-4 homologue, plays a crucial role in mitochondrial mediated apoptosis. To determine whether a mutant Apaf-1 is involved in the pathogenesis of malignant lymphomas, we performed sequence analysis of its transcriptional gene product. Therefore, cDNAs coding for Apaf-1 were examined in six lymphoma derived cell lines, in three non-lymphoid tumor cell lines, as well as in peripheral blood lymphocytes and in tissue from heart, kidney, and liver by RT-PCR. These studies did not disclose the expected tumor related alterations but led instead to the identification of three novel forms of Apaf-1. These forms vary in length, but contain all functional domains formerly characterized in the Apaf-1 protein. The results indicate that the previously published sequence is not the Apaf-1 form most widely expressed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Apoptosis
  • Apoptotic Protease-Activating Factor 1
  • Hodgkin Disease / genetics
  • Humans
  • Lymphocytes / chemistry
  • Lymphoma / genetics
  • Molecular Sequence Data
  • Mutation
  • Proteins / analysis
  • Proteins / chemistry
  • Proteins / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Tumor Cells, Cultured

Substances

  • APAF1 protein, human
  • Apoptotic Protease-Activating Factor 1
  • Proteins

Associated data

  • GENBANK/AJ243011
  • GENBANK/AJ243048
  • GENBANK/AJ243107