Induction of humoral and cellular immune responses against the nucleocapsid of bovine viral diarrhea virus by an adenovirus vector with an inducible promoter

Virology. 1999 Aug 15;261(1):1-7. doi: 10.1006/viro.1999.9828.


A new recombinant adenovirus was constructed that expressed the nucleocapsid (C protein or p14) of the bovine viral diarrhea virus (BVDV) under the control of a tetracycline-regulatable promoter. Mice covaccinated with this recombinant adenovirus, accompanied by another recombinant adenovirus expressing the trans-activator protein, induced a strong humoral immune response to the BVDV/C protein as detected by ELISA. Splenocytes from mice immunized with the recombinant adenovirus showed a specific proliferation response to both genotypes (type 1 and 2) of BVDV. High levels of IFN-gamma were detected in the supernatant of murine mononuclear cells of mice immunized by the recombinant adenovirus when stimulated in vitro by both genotypes of BVDV. These results indicate that this recombinant adenovirus is highly immunogenic and stimulates both cellular and humoral immune responses against the nucleocapsid of BVDV.

MeSH terms

  • Adenoviruses, Human / genetics*
  • Adenoviruses, Human / immunology
  • Animals
  • Antibodies, Viral / blood*
  • Cattle
  • Diarrhea Viruses, Bovine Viral / chemistry
  • Diarrhea Viruses, Bovine Viral / immunology*
  • Genetic Vectors
  • Immunity, Cellular*
  • Interferon-gamma / biosynthesis
  • Leukocytes, Mononuclear / immunology
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred BALB C
  • Nucleocapsid Proteins / genetics
  • Nucleocapsid Proteins / immunology*
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins / administration & dosage
  • Recombinant Fusion Proteins / immunology
  • Tetracycline / pharmacology
  • Vaccination
  • Vaccines, Synthetic / immunology*
  • Viral Vaccines / immunology


  • Antibodies, Viral
  • Nucleocapsid Proteins
  • Recombinant Fusion Proteins
  • Vaccines, Synthetic
  • Viral Vaccines
  • Interferon-gamma
  • Tetracycline