Two eosinophil granule proteins, eosinophil peroxidase and major basic protein, inhibit mucin release from hamster tracheal surface epithelial cells in primary culture

Inflamm Res. 1999 Jun;48(6):314-7. doi: 10.1007/s000110050466.


Objectives and design: Effects of eosinophil proteins on airway goblet cell mucin release were investigated using primary hamster tracheal surface epithelial (HTSE) cell cultures.

Materials and methods: HTSE cells were metabolically labeled using 3H-glucosamine and chased in the presence of varying concentrations of eosinophil proteins. The amount of 3H-mucin in the spent media was measured by Sepharose CL-4B gel-filtration column chromatography. Possible cytotoxicity by the eosinophil proteins was assessed by measurements of both lactic dehydrogenase and mucin release during as well as after the treatment period.

Results: (1) Neither eosinophil cationic protein nor eosinophil-derived neurotoxin affected mucin release at concentrations up to 10(-6) M; (2) both major basic protein (MBP) and eosinophil peroxidase (EPO) inhibited mucin release in a dose-dependent fashion, and the inhibitory effect by these proteins appeared to be reversible; (3) neither MBP nor EPO caused any apparent cytotoxicity at concentrations up to 10(-6) M.

Conclusion: Eosinophil proteins such as MBP and EPO inhibit mucin release from primary HTSE cells without causing any apparent cytotoxicity.

MeSH terms

  • Animals
  • Blood Proteins / pharmacology*
  • Cells, Cultured
  • Cricetinae
  • Eosinophil Granule Proteins
  • Eosinophil Peroxidase
  • Eosinophils* / enzymology
  • Epithelium / metabolism
  • Mesocricetus
  • Mucins / drug effects*
  • Mucins / metabolism
  • Peroxidases / pharmacology*
  • Ribonucleases*
  • Trachea / drug effects*
  • Trachea / metabolism


  • Blood Proteins
  • Eosinophil Granule Proteins
  • Mucins
  • Eosinophil Peroxidase
  • Peroxidases
  • Ribonucleases