This study extends previous work on exit jet particle velocity in the in vivo canine model of phonation by measuring air particle velocity at multiple locations in the midline of the glottis and across multiple levels of recurrent laryngeal nerve (RLN) and superior laryngeal nerve (SLN) stimulation. In a second experiment, exit jet particle velocity was measured at midline and offmidline positions with constant levels of RLN and SLN stimulation. In this study, peak particle velocity was higher at the anterior commissure than at the posterior commissure in the midline of the glottis, and peak particle velocity was higher at the midline than at offmidline positions. In addition, increasing levels of RLN stimulation resulted in increasing peak particle velocity; however, increasing levels of SLN stimulation failed to produce a uniform effect on peak particle velocity.