JIL-1: a novel chromosomal tandem kinase implicated in transcriptional regulation in Drosophila

Mol Cell. 1999 Jul;4(1):129-35. doi: 10.1016/s1097-2765(00)80195-1.

Abstract

We have cloned and characterized JIL-1, a novel tandem kinase in Drosophila that associates with the chromosomes throughout the cell cycle. Antibody staining and live imaging of JIL-1-GFP transgenic flies show that JIL-1 localizes to the gene-rich interband regions of larval polytene chromosomes and is upregulated almost 2-fold on the hypertranscribed male X chromosome compared to autosomes. Phylogenetic analysis suggests that JIL-1 together with human MSKs defines a separate family of tandem kinases. That JIL-1 is a functional kinase was demonstrated by autophosphorylation and phosphorylation of histone H3 in vitro. Based on these findings, we propose that JIL-1 may play a role in transcriptional control potentially by regulating chromatin structure.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Cell Cycle
  • Chromatin / chemistry
  • Chromosomal Proteins, Non-Histone / chemistry
  • Chromosomal Proteins, Non-Histone / genetics*
  • Cloning, Molecular
  • Drosophila / embryology
  • Drosophila / enzymology*
  • Gene Expression Regulation
  • Histones / metabolism
  • Humans
  • Insect Proteins / chemistry
  • Insect Proteins / genetics
  • Male
  • Molecular Sequence Data
  • Phylogeny
  • Protein Kinases / chemistry
  • Protein Kinases / genetics*
  • Salivary Glands / enzymology
  • X Chromosome / genetics

Substances

  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • Histones
  • Insect Proteins
  • Protein Kinases

Associated data

  • GENBANK/AF142061