Dual inhibitory effects of dimethyl sulfoxide on poly(ADP-ribose) synthetase

J Enzyme Inhib. 1999;14(3):239-50. doi: 10.3109/14756369909030319.

Abstract

Dimethyl sulfoxide (DMSO), a solvent popularly used for dissolving water-insoluble compounds, is a weak inhibitor of poly(ADP-ribose) synthetase, that is a nuclear enzyme producing (ADP-ribose)n from NAD+. The inhibitory mode and potency depend on the concentration of substrate, NAD+, as well as the temperature of the reaction; at micromolar concentrations of NAD+, the inhibition by DMSO is biphasic at 37 degrees C, but is monophasic and apparently competitive with NAD+ at 25 degrees C. DMSO, on the other hand, diminishes dose-dependently and markedly the inhibitory potency of benzamide and other inhibitors. Other organic solvents, ethanol and methanol, also show a biphasic effect on the synthetase activity at different concentrations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzamides / pharmacology
  • Dimethyl Sulfoxide / pharmacology*
  • Ethanol / pharmacology
  • Methanol / pharmacology
  • NAD / pharmacology
  • Poly(ADP-ribose) Polymerase Inhibitors*
  • Solubility
  • Solvents
  • Water

Substances

  • Benzamides
  • Poly(ADP-ribose) Polymerase Inhibitors
  • Solvents
  • Water
  • NAD
  • Ethanol
  • benzamide
  • Methanol
  • Dimethyl Sulfoxide