X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli

Science. 1999 Aug 13;285(5430):1061-6. doi: 10.1126/science.285.5430.1061.

Abstract

Type 1 pili-adhesive fibers expressed in most members of the Enterobacteriaceae family-mediate binding to mannose receptors on host cells through the FimH adhesin. Pilus biogenesis proceeds by way of the chaperone/usher pathway. The x-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli at 2.5 angstrom resolution reveals the basis for carbohydrate recognition and for pilus assembly. The carboxyl-terminal pilin domain of FimH has an immunoglobulin-like fold, except that the seventh strand is missing, leaving part of the hydrophobic core exposed. A donor strand complementation mechanism in which the chaperone donates a strand to complete the pilin domain explains the basis for both chaperone function and pilus biogenesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adhesins, Bacterial / chemistry*
  • Adhesins, Bacterial / metabolism
  • Adhesins, Escherichia coli*
  • Amino Acid Sequence
  • Bacterial Outer Membrane Proteins / chemistry*
  • Bacterial Outer Membrane Proteins / metabolism
  • Bacterial Proteins*
  • Chlorpropamide / analogs & derivatives
  • Chlorpropamide / metabolism
  • Crystallography, X-Ray
  • Escherichia coli / chemistry*
  • Escherichia coli / metabolism
  • Escherichia coli / pathogenicity
  • Escherichia coli Proteins*
  • Fimbriae Proteins
  • Fimbriae, Bacterial / chemistry
  • Fimbriae, Bacterial / metabolism*
  • Fimbriae, Bacterial / ultrastructure
  • Hydrogen Bonding
  • Membrane Proteins / chemistry*
  • Models, Molecular
  • Molecular Chaperones / chemistry*
  • Molecular Chaperones / metabolism
  • Molecular Sequence Data
  • Protein Conformation
  • Protein Folding
  • Protein Structure, Secondary
  • Sequence Alignment

Substances

  • Adhesins, Bacterial
  • Adhesins, Escherichia coli
  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Escherichia coli Proteins
  • Membrane Proteins
  • Molecular Chaperones
  • cyclohexylbutanoyl-N-hydroxyethylglucamide
  • fimC protein, E coli
  • fimH protein, E coli
  • Fimbriae Proteins
  • Chlorpropamide

Associated data

  • PDB/1QUN