A novel quantitative multiplex NASBA method: application to measuring tissue factor and CD14 mRNA levels in human monocytes

Nucleic Acids Res. 1999 Sep 1;27(17):e15. doi: 10.1093/nar/27.17.e15.


A new method to quantify two individual mRNAs in a single NASBA reaction is described. In this study, tissue factor and CD14 mRNAs were used as a model system. RNA ratios of -4 to +4 log units were determined with good precision (within 0.3 log) and accuracy (within 0.2 log). By measuring both mRNAs in human monocytes that were stimulated with LPS, the multiplex Q-NASBA proved to be a successful tool to monitor the expression levels of two individual mRNAs in a single-tube amplification system. The method has potential in all fields in which quantitative information is needed on two individual RNAs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Assay / methods
  • Cells, Cultured
  • Genetic Markers
  • Humans
  • Lipopolysaccharide Receptors / genetics*
  • Lipopolysaccharides / immunology
  • Monocytes / immunology
  • Monocytes / metabolism*
  • Nucleic Acid Amplification Techniques*
  • RNA, Messenger / analysis*
  • Reproducibility of Results
  • Thromboplastin / genetics*
  • Time Factors


  • Genetic Markers
  • Lipopolysaccharide Receptors
  • Lipopolysaccharides
  • RNA, Messenger
  • Thromboplastin