Characterization of myosin V from PC12 cells

Biochem Biophys Res Commun. 1999 Aug 19;262(1):98-102. doi: 10.1006/bbrc.1999.1171.


PC12 cell line is a cellular model to study neurite outgrowth and neurotransmitter release mechanisms. Molecular motors may be involved in these responses and myosin V could be a candidate to mediate these effects. Overlay experiments using [(125)I]-calmodulin showed that PC12 cells possess several calmodulin-binding proteins, some of them around 190-210 kDa. Western blots using affinity purified polyclonal antibodies raised against chicken brain myosin V revealed a component of 190 kDa, a molecular mass typical of myosin V. Furthermore, Northern blots using a myosin V probe also detected a transcript of around 12 kbp. Immunofluorescence cytochemistry demonstrated the localization of myosin V throughout the cytoplasm, in the neurites, growth cone tips, and with an intense asymmetrical perinuclear labeling. Western blot analyses of PC12 cellular extracts after FGF-2 and/or dibutyryl cAMP treatment revealed variations between myosin V and myosin II expression during neuronal differentiation. These results demonstrated the presence of myosin V in PC12 cells and also suggest a role for this motor molecule in the neuronal differentiation response in PC12 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Bucladesine / pharmacology
  • Calmodulin / metabolism
  • Calmodulin-Binding Proteins / genetics
  • Calmodulin-Binding Proteins / metabolism*
  • Cell Differentiation / drug effects
  • Cell Nucleus / drug effects
  • Cell Nucleus / metabolism
  • Cytoplasm / drug effects
  • Cytoplasm / metabolism
  • Fibroblast Growth Factor 2 / pharmacology
  • Fluorescent Antibody Technique
  • Gene Expression Regulation / drug effects
  • Growth Cones / drug effects
  • Growth Cones / metabolism
  • Molecular Weight
  • Myosin Type V*
  • Myosins / metabolism
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Neurites / drug effects
  • Neurites / metabolism
  • Neurons / cytology*
  • Neurons / drug effects
  • Neurons / metabolism
  • PC12 Cells
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Rats


  • Calmodulin
  • Calmodulin-Binding Proteins
  • Nerve Tissue Proteins
  • RNA, Messenger
  • chicken brain myosin-V p190
  • Fibroblast Growth Factor 2
  • Bucladesine
  • Myosin Type V
  • Myosins