Role of Toxoplasma gondii HSP70 and Toxoplasma gondii HSP30/bag1 in antibody formation and prophylactic immunity in mice experimentally infected with Toxoplasma gondii

Microbiol Immunol. 1999;43(5):471-9. doi: 10.1111/j.1348-0421.1999.tb02430.x.

Abstract

Production of antibodies against Toxoplasma gondii (T. gondii)-derived stress proteins, T. gondii HSP70 (T.g.HSP70) and T.g.HSP30/bagl, in C57BL/6 and BALB/c mice perorally infected with cysts of the avirulent Fukaya strain of T. gondii was analyzed. Production of anti-T.g.HSP70 IgG antibodies was transient, whereas production of anti-T.g.HSP30/bag1 IgG antibodies persisted after infection in both C57BL/6 and BALB/c mice. C57BL/6 mice, a susceptible strain, predominantly produced IgG antibodies specific for T.g.HSP70, whereas BALB/c mice, a resistant strain, predominantly produced IgG antibodies specific for T.g.HSP30/bag1, after T. gondii infection. Immunization with rT.g.HSP30/bag1 enhanced, whereas immunization with rT.g.HSP70 reduced host protective immunity against T. gondii infection with a cyst-forming avirulent strain, Fukaya, and a virulent strain, RH.

MeSH terms

  • Animals
  • Antibodies, Protozoan / blood
  • Antigens, Protozoan*
  • Female
  • H-2 Antigens
  • HSP30 Heat-Shock Proteins
  • HSP70 Heat-Shock Proteins / immunology
  • Heat-Shock Proteins / immunology*
  • Immunoglobulin G / blood
  • Membrane Proteins / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Protozoan Proteins / immunology
  • Protozoan Vaccines
  • Toxoplasma / immunology*
  • Toxoplasmosis, Animal / mortality
  • Toxoplasmosis, Animal / prevention & control*
  • Vaccination*

Substances

  • Antibodies, Protozoan
  • Antigens, Protozoan
  • BAG1 protein, Toxoplasma
  • H-2 Antigens
  • HSP30 Heat-Shock Proteins
  • HSP70 Heat-Shock Proteins
  • Heat-Shock Proteins
  • Immunoglobulin G
  • Membrane Proteins
  • Protozoan Proteins
  • Protozoan Vaccines
  • SAG1 antigen, Toxoplasma