Detection of anti-annexin IV and V antibodies in patients with antiphospholipid syndrome and systemic lupus erythematosus

J Rheumatol. 1999 Aug;26(8):1715-20.


Objective: Annexins (Anx) are a family of structurally related proteins that bind to phospholipids in a calcium dependent manner. It has been reported that antibodies to Anx V, which acts as an antithrombotic protein, are associated with thrombosis in systemic lupus erythematosus (SLE) and/or antiphospholipid syndrome (APS). Homology between the primary structures of Anx IV and Anx V is the highest among members of the Anx family. We investigated whether anti-Anx IV autoantibodies can be detected in the sera of patients with SLE and/or APS.

Methods: Seventy-four patients with SLE/APS were divided into 3 groups: Group A, patients with SLE but no clinical or serological features of APS; Group B, patients with SLE having only serological signs of APS; and Group C, patients with clinical symptoms and serological signs of APS. Anx IV and Anx V were prepared by recombinant technique. Anti-Anx IV, Anx V, cardiolipin (CL), and CL beta2-glycoprotein I were detected by ELISA.

Results: Anti-Anx IV was found in 15.4% of Group A, 20.0% of Group B, and 21.7% of Group C. Anti-Anx V was found in 3.8% of Group A, 28.0% of Group B, and 30.4% of Group C. Significant correlations were noted between anti-Anx IV titer and anti-Anx V titer (p<0.001), and between anti-Anx IV titer and aCL titer (p<0.01).

Conclusion: Anti-Anx IV and V antibodies were characterized in the sera of patients with SLE/APS. Significantly higher frequency of arterial or venous thrombosis was found in patients with anti-Anx V.

MeSH terms

  • Adolescent
  • Adult
  • Annexin A4 / immunology*
  • Annexin A5 / immunology*
  • Antiphospholipid Syndrome / blood
  • Antiphospholipid Syndrome / immunology*
  • Autoantibodies / analysis*
  • Autoantibodies / blood
  • Binding, Competitive / drug effects
  • Cardiolipins / immunology
  • Female
  • Glycoproteins / immunology
  • Humans
  • Lupus Erythematosus, Systemic / blood
  • Lupus Erythematosus, Systemic / immunology*
  • Male
  • Middle Aged
  • Phosphatidylserines / pharmacology
  • beta 2-Glycoprotein I


  • Annexin A4
  • Annexin A5
  • Autoantibodies
  • Cardiolipins
  • Glycoproteins
  • Phosphatidylserines
  • beta 2-Glycoprotein I