Structure and alternative splicing of the gene encoding alpha1I, a human brain T calcium channel alpha1 subunit

Neurosci Lett. 1999 Jul 16;269(3):121-4. doi: 10.1016/s0304-3940(99)00319-5.


The structure of CACNA1I, the gene encoding alpha1I, a human brain T Ca2+ channel alpha1 subunit, was determined by comparison of polymerase chain reaction-amplified brain cDNA and genomic sequences. The gene consists of at least 36 exons spanning at least 115,168 basepairs of chromosome 22q12.3-13.2. The predicted protein has 2016 amino acids and 28 potential phosphorylation sites. Alternative splicing of the gene occurs at two sites: cassette exon 9 and an alternative acceptor in exon 33. Molecular diversity generated by alternative splicing and post-translational modification of this and other members of the T alpha1 subunit gene family may account for the observed heterogeneity of T currents in central neurons.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing*
  • Brain / metabolism*
  • Calcium Channels / chemistry
  • Calcium Channels / genetics*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 22*
  • DNA, Complementary
  • Exons
  • Glycosylation
  • Humans
  • Introns
  • Multigene Family
  • Mutagenesis, Insertional
  • Phosphorylation
  • Polymerase Chain Reaction
  • Protein Structure, Secondary


  • Calcium Channels
  • DNA, Complementary

Associated data

  • GENBANK/AF129133