Minichromosome maintenance as a genetic assay for defects in DNA replication

Methods. 1999 Jul;18(3):329-34. doi: 10.1006/meth.1999.0793.

Abstract

Minichromosome maintenance (mcm) is an effective genetic assay for mutants defective in DNA replication. Two classes of mcm mutants have been identified using this screen: those that differentially affect the activities of certain autonomously replicating sequences (ARSs) and those that uniformly affect the activities of all ARSs. The ARS-specific MCM genes are essential for the initiation of DNA replication. Among these are members of the MCM2-7 family that encode subunits of the preinitiation complex and MCM10, whose gene product interacts with members of the Mcm2-7 proteins. Among the ARS-nonspecific MCM gene products are chromosome transmission factors. Refinement of this genetic assay as a screening tool and further analysis of existing mcm mutants may reveal new replication initiation proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosomal Proteins, Non-Histone
  • Chromosomes, Fungal*
  • DNA Replication / genetics*
  • Fungal Proteins / genetics
  • Genetic Complementation Test
  • Genetic Techniques
  • Mutation
  • Phenotype
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins

Substances

  • Chromosomal Proteins, Non-Histone
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • MCM2 protein, S cerevisiae