Alternative splicing generates two isoforms of the beta3-adrenoceptor which are differentially expressed in mouse tissues

Br J Pharmacol. 1999 Jul;127(6):1525-31. doi: 10.1038/sj.bjp.0702688.


The beta3-adrenoceptor (AR) differs from the beta1-AR and beta2-ARs in having introns within and downstream of the coding block. This study demonstrates two splice variants of the mouse beta3-AR which differ within the coding region. Reverse transcription/polymerase chain reaction with intron-spanning primers was used to demonstrate the splice variant of the mouse beta3-adrenoceptor. The novel beta3b-AR has 17 amino acids encoded by exon 2 (SSLLREPRHLYTCLGYP) which differ from the 13 in the known beta3a-AR (RFDGYEGARPFPT). Beta3b-AR mRNA is differentially expressed in mouse tissues, with levels relative to beta3a-AR mRNA highest in hypothalamus, cortex and white adipose tissue, and lower in ileum smooth muscle and brown adipose tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / metabolism
  • Alternative Splicing*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cerebral Cortex / metabolism
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Gene Expression Regulation
  • Hypothalamus / metabolism
  • Ileum / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Muscle, Smooth / metabolism
  • Protein Isoforms / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Adrenergic, beta / genetics*
  • Receptors, Adrenergic, beta-3
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Tissue Distribution


  • DNA, Complementary
  • Protein Isoforms
  • RNA, Messenger
  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic, beta-3

Associated data

  • GENBANK/AF193027