A Drosophila Axin homolog, Daxin, inhibits Wnt signaling

Development. 1999 Sep;126(18):4165-73.

Abstract

The vertebrate Axin protein, the product of the mouse fused gene, binds to beta-catenin to inhibit Wnt signaling. We have identified a homolog of Axin in Drosophila, Daxin. Using double-stranded RNA interference, we generated loss-of-function phenotypes that are similar to overexpression of the Drosophila Wnt gene wingless (wg). Overexpression of Daxin produces phenotypes similar to loss of wg. In addition, we show that Daxin overexpression can modify phenotypes elicited by wg and another Drosophila Wnt gene, DWnt-2. Using immunoprecipitation of endogenous Daxin protein from embryos we show that Daxin interacts with Armadillo and Zeste-white 3. The loss-of-function and overexpression phenotypes show that Daxin, like its mammalian counterpart, acts as a negative regulator of wg/Wnt signaling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Amino Acid Sequence
  • Animals
  • Armadillo Domain Proteins
  • Axin Protein
  • Base Sequence
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism*
  • Drosophila Proteins*
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Embryo, Nonmammalian
  • Expressed Sequence Tags
  • Eye / growth & development
  • Eye Abnormalities / genetics
  • Female
  • Gene Expression Regulation, Developmental
  • Genetic Techniques
  • Glycogen Synthase Kinase 3*
  • Insect Proteins / genetics
  • Insect Proteins / metabolism
  • Molecular Sequence Data
  • Ovary / growth & development
  • Ovary / metabolism
  • Protein-Serine-Threonine Kinases / genetics
  • Protein-Serine-Threonine Kinases / metabolism
  • Proteins / genetics
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism*
  • RNA / genetics
  • Repressor Proteins*
  • Sequence Homology, Amino Acid
  • Signal Transduction*
  • Trans-Activators*
  • Transcription Factors
  • Wings, Animal / growth & development
  • Wnt1 Protein
  • Wnt2 Protein

Substances

  • ARM protein, Drosophila
  • Adaptor Proteins, Signal Transducing
  • Armadillo Domain Proteins
  • Axin Protein
  • Axn protein, Drosophila
  • Carrier Proteins
  • Drosophila Proteins
  • Insect Proteins
  • Proteins
  • Proto-Oncogene Proteins
  • Repressor Proteins
  • Trans-Activators
  • Transcription Factors
  • Wnt1 Protein
  • Wnt2 Protein
  • Wnt2 protein, Drosophila
  • wg protein, Drosophila
  • RNA
  • Protein-Serine-Threonine Kinases
  • Sgg protein, Drosophila
  • Glycogen Synthase Kinase 3