Gene structure of the murine NK cell receptor 2B4: presence of two alternatively spliced isoforms with distinct cytoplasmic domains

Eur J Immunol. 1999 Aug;29(8):2392-9. doi: 10.1002/(SICI)1521-4141(199908)29:08<2392::AID-IMMU2392>3.0.CO;2-R.


The NK cell receptor 2B4 is expressed on the surface of all murine NK cells and a subset of T cells. Ligation of 2B4 with monoclonal antibodies increases target cell lysis and IFN-gamma production. 2B4 is the high-affinity counter-receptor for CD48 in mice and humans. 2B4-L is a member of the CD2 subgroup of the immunoglobulin supergene family, which includes CD48, LFA-3, CD84, Ly9 and SLAM. Here we describe 2B4-S, a second 2B4 isoform, and the genomic structure of the 2B4 gene. 2B4-S is identical to the 5' end of 2B4-L, differing only at the 3' end, corresponding to a portion of the cytoplasmic domain and the 3' untranslated sequence. Both 2B4-L and 2B4-S are expressed on IL-2-activated NK cells. The genomic clone of 2B4 reveals that the two cDNA clones are products of alternative splicing. Since they differ only in a portion of the cytoplasmic domain, it is likely that they transduce different signals.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Antigens, CD*
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • Cytoplasm / chemistry
  • DNA Primers / genetics
  • DNA, Complementary / genetics
  • Exons
  • Humans
  • Introns
  • Killer Cells, Natural / immunology*
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / genetics*
  • Membrane Glycoproteins / immunology*
  • Mice
  • Molecular Sequence Data
  • Protein Isoforms / chemistry
  • Protein Isoforms / genetics
  • Protein Isoforms / immunology
  • Receptors, Immunologic / chemistry
  • Receptors, Immunologic / genetics*
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Signaling Lymphocytic Activation Molecule Family


  • Antigens, CD
  • CD244 protein, human
  • Cd244a protein, mouse
  • DNA Primers
  • DNA, Complementary
  • Membrane Glycoproteins
  • Protein Isoforms
  • Receptors, Immunologic
  • Signaling Lymphocytic Activation Molecule Family