Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells

FEBS Lett. 1999 Aug 13;456(3):379-83. doi: 10.1016/s0014-5793(99)00984-9.

Abstract

Goniothalamin, a plant styrylpyrone derivative isolated from Goniothalamus andersonii, induced apoptosis in Jurkat T-cells as assessed by the externalisation of phosphatidylserine. Immunoblotting showed processing of caspases-3 and -7 with the appearance of their catalytically active large subunits of 17 and 19 kDa, respectively. Activation of these caspases was further evidenced by detection of poly(ADP-ribose) polymerase cleavage (PARP). Pre-treatment with the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp fluoromethyl ketone (Z-VAD.FMK) blocked apoptosis and the resultant cleavage of these caspases and PARP. Our results demonstrate that activation of at least two effector caspases is a key feature of goniothalamin-induced apoptosis in Jurkat T-cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Chloromethyl Ketones / pharmacology
  • Annexin A5 / analysis
  • Annexin A5 / metabolism
  • Apoptosis / drug effects*
  • Caspase 3
  • Caspase 7
  • Caspase Inhibitors
  • Caspases / metabolism*
  • Cysteine Proteinase Inhibitors / pharmacology
  • Enzyme Activation / drug effects
  • Humans
  • Jurkat Cells / drug effects*
  • Jurkat Cells / enzymology
  • Jurkat Cells / pathology
  • Poly(ADP-ribose) Polymerases / metabolism
  • Pyrones / metabolism
  • Pyrones / pharmacology*

Substances

  • Amino Acid Chloromethyl Ketones
  • Annexin A5
  • Caspase Inhibitors
  • Cysteine Proteinase Inhibitors
  • Pyrones
  • benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone
  • goniothalamin
  • Poly(ADP-ribose) Polymerases
  • CASP3 protein, human
  • CASP7 protein, human
  • Caspase 3
  • Caspase 7
  • Caspases