Interleukin-1 receptor antagonist mRNA expression and the progression of gastric carcinoma

Cancer Lett. 1999 Aug 3;142(2):179-84. doi: 10.1016/s0304-3835(99)00162-7.


Interleukin-1 receptor antagonist (IL-1ra), an endogeneous inhibitor of IL-1, plays an immunosuppressive role in vivo by blocking the proinflammatory effects of IL-1. In the present study, we examined whether IL-1ra expression in human gastric carcinoma correlates with tumor progression and/or metastatic potential. The reverse transcription-polymerase chain reaction was used to compare the expression of the secreted form of IL-1ra (sIL-1ra) and the intracellular form of IL-1ra (icIL-1ra) mRNA in tumor and corresponding benign tissue obtained from 38 patients with gastric carcinoma. The incidence of sIL-1ra mRNA expression was significantly higher in tumor (52%) than in corresponding benign tissue (18%) (P = 0.002). On the contrary, icIL-1ra mRNA was detected in all tumors and benign tissues. The expression of sIL-1ra mRNA by malignant tissue correlated positively with both lymph node metastasis (P = 0.008) and liver metastasis (P = 0.015). There was no association between tumor sIL-lra mRNA expression and other clinicopathologic factors. The degree of regional lymph node reaction, such as sinus histiocytosis, in tumors expressing sI-1ra mRNA was significantly weaker than that in tumors without sIL-1ra mRNA expression (5/20 vs. 12/18, P = 0.010). These results demonstrate that the altered expression of sIL-1ra by malignant tissue may be related to the progression of gastric carcinoma via modulating host immune response.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Female
  • Humans
  • Interleukin 1 Receptor Antagonist Protein
  • Liver Neoplasms / secondary
  • Lymphatic Metastasis
  • Male
  • Middle Aged
  • RNA, Messenger / biosynthesis*
  • Receptors, Interleukin-1 / antagonists & inhibitors
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sialoglycoproteins / genetics*
  • Stomach Neoplasms / metabolism*
  • Stomach Neoplasms / pathology


  • IL1RN protein, human
  • Interleukin 1 Receptor Antagonist Protein
  • RNA, Messenger
  • Receptors, Interleukin-1
  • Sialoglycoproteins