A simple method for constructing E1- and E1/E4-deleted recombinant adenoviral vectors

Hum Gene Ther. 1999 Aug 10;10(12):2013-7. doi: 10.1089/10430349950017374.


We previously developed a two-plasmid in vitro ligation method that did not require a recombination step to produce new recombinant E1- or E1/E3-deleted adenoviral vectors. In this study, we have modified the system to improve the simplicity of vector construction and, in addition, to allow for production of an E1/E4-deleted vector.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae / genetics*
  • Adenovirus E1 Proteins / genetics*
  • Adenovirus E3 Proteins / genetics
  • Adenovirus E4 Proteins / genetics*
  • Gene Deletion
  • Genetic Vectors / genetics*
  • Plasmids / genetics
  • Recombinant Proteins / genetics*
  • beta-Galactosidase / metabolism


  • Adenovirus E1 Proteins
  • Adenovirus E3 Proteins
  • Adenovirus E4 Proteins
  • Recombinant Proteins
  • beta-Galactosidase