P53 deletion is not a frequent event in multiple myeloma

Br J Haematol. 1999 Sep;106(3):717-9. doi: 10.1046/j.1365-2141.1999.01615.x.


Recently a high incidence of interstitial deletion of the P53 locus has been reported in multiple myeloma (MM) patients. Considering the importance of such an event, we analysed 79 patients with advanced-stage disease using fluorescence in situ hybridization (FISH). Strikingly, we found only 7/79 patients with a P53 deletion. In order to rule out any differences in probe selection, we reanalysed all the patients with the same probe as that used in a previous study, and confirmed the low incidence of P53 deletion (7/79, 9%). The only explanation is a difference in hybridization efficiency. Since hybridization is far less efficient on malignant plasma cells than on other bone marrow cells we suggest that this poor hybridization efficiency may lead to a false P53 deletion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Deletion*
  • Genes, p53 / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Multiple Myeloma / genetics*