Inhibition of dextran sulphate sodium (DSS)-induced colitis in mice by intracolonically administered antibodies against adhesion molecules (endothelial leucocyte adhesion molecule-1 (ELAM-1) or intercellular adhesion molecule-1 (ICAM-1))

Clin Exp Immunol. 1999 Sep;117(3):462-8. doi: 10.1046/j.1365-2249.1999.00985.x.


We examined the effect of intracolonic administration of anti-adhesion molecule antibodies on DSS-induced colitis in mice. Immunohistochemical staining in mice with colitis showed increased expression of ELAM-1 and ICAM-1 on endothelial cells of vessels in the lamina propria and submucosa at sites of inflamed lesions. Intracolonic administration of anti-ELAM-1 or anti-ICAM-1 antibody decreased bloody stools, anaemia, and histologically evident damage, as well as myeloperoxidase activity and IL-1beta content. We concluded that adhesion molecule expression is important in the development of DSS-induced colitis in mice and that intracolonic administration of anti-adhesion molecule antibodies, especially anti-ELAM-1 antibody, effectively inhibits the colonic inflammation. Intracolonic administration of anti-adhesion molecule antibodies may show therapeutic promise in ulcerative colitis.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Monoclonal / therapeutic use
  • Colitis, Ulcerative / chemically induced
  • Colitis, Ulcerative / immunology*
  • Colitis, Ulcerative / pathology
  • Colitis, Ulcerative / therapy
  • Dextran Sulfate / antagonists & inhibitors
  • Dextran Sulfate / pharmacology
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • E-Selectin / biosynthesis
  • E-Selectin / immunology*
  • Female
  • Immunotherapy
  • Intercellular Adhesion Molecule-1 / biosynthesis
  • Intercellular Adhesion Molecule-1 / immunology*
  • Interleukin-1 / analysis
  • Mice
  • Mice, Inbred BALB C
  • Peroxidase / metabolism


  • Antibodies, Monoclonal
  • E-Selectin
  • Interleukin-1
  • Intercellular Adhesion Molecule-1
  • Dextran Sulfate
  • Peroxidase