Protein delipidation and precipitation by tri-n-butylphosphate, acetone, and methanol treatment for isoelectric focusing and two-dimensional gel electrophoresis

Anal Biochem. 1999 Sep 10;273(2):313-5. doi: 10.1006/abio.1999.4224.
No abstract available

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetone
  • Animals
  • Buffers
  • Chemical Precipitation
  • Decapoda
  • Egg Proteins / isolation & purification
  • Electrophoresis, Gel, Two-Dimensional / methods*
  • Female
  • Isoelectric Focusing / methods*
  • Lipids / isolation & purification*
  • Methanol
  • Organophosphates
  • Proteins / isolation & purification*
  • Solubility
  • Solvents

Substances

  • Buffers
  • Egg Proteins
  • Lipids
  • Organophosphates
  • Proteins
  • Solvents
  • Acetone
  • tributyl phosphate
  • Methanol