Transcriptional regulation of C/EBPdelta in G(0) growth-arrested mouse mammary epithelial cells

Biochem Biophys Res Commun. 1999 Sep 7;262(3):696-701. doi: 10.1006/bbrc.1999.1256.

Abstract

Little is known about the control of cell cycle exit/G(0) entry, or the regulation of genes that are expressed during G(0). In this report we used primer extension analysis to demonstrate the high level of C/EBPdelta mRNA expression in G(0) growth-arrested HC11 mouse mammary epithelial cells and to identify the C/EBPdelta transcription start site. The C/EBPdelta gene transcription rate and promoter activity are both highly induced in G(0) growth-arrested HC11 cells. The C/EBPdelta gene promoter also exhibits G(0)-specific autoregulation. In contrast, the C/EBPdelta promoter activity decreases in G(0) growth-arrested NIH 3T3 cells. These data indicate that C/EBPdelta is among a relatively small number of genes actively transcribed during G(0) growth arrest. C/EBPdelta may regulate the expression of genes implicated in the initiation or maintenance of mammary epithelial cell G(0) growth arrest.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Animals
  • Base Sequence
  • CCAAT-Enhancer-Binding Protein-delta
  • CCAAT-Enhancer-Binding Proteins*
  • Cell Line
  • DNA-Binding Proteins / genetics*
  • Epithelial Cells / cytology*
  • Epithelial Cells / metabolism*
  • Female
  • Gene Expression Regulation*
  • Homeostasis
  • Mammary Glands, Animal / cytology*
  • Mammary Glands, Animal / metabolism
  • Mice
  • Molecular Sequence Data
  • Nuclear Proteins / genetics*
  • Promoter Regions, Genetic*
  • RNA, Messenger / genetics
  • Resting Phase, Cell Cycle
  • Transcription Factors / genetics*
  • Transcription, Genetic*

Substances

  • CCAAT-Enhancer-Binding Proteins
  • Cebpd protein, mouse
  • DNA-Binding Proteins
  • Nuclear Proteins
  • RNA, Messenger
  • Transcription Factors
  • CCAAT-Enhancer-Binding Protein-delta