VanE, a new type of acquired glycopeptide resistance in Enterococcus faecalis BM4405

Antimicrob Agents Chemother. 1999 Sep;43(9):2161-4. doi: 10.1128/AAC.43.9.2161.


Enterococcus faecalis BM4405 was resistant to low levels of vancomycin (MIC, 16 microg/ml) and was susceptible to teicoplanin (MIC, 0.5 microg/ml). No PCR product was obtained when the total DNA of this clinical isolate was used as a template with primers specific for glycopeptide resistance genes vanA, vanB, vanC, and vanD. However, a 604-bp PCR fragment was obtained when V1 and V2 degenerate primers were used and total DNA was digested with HindIII as a template. The product was cloned and sequenced. The deduced amino acid sequence had greater identity (55%) with VanC than with VanA (45%), VanB (43%), or VanD (44%). This was consistent with the fact that BM4405 synthesized peptidoglycan precursors that terminated in D-serine residues. After induction with vancomycin, weak D,D-dipeptidase and penicillin-insensitive D,D-carboxypeptidase activities were detected in cytoplasmic extracts of BM4405, whereas a serine racemase activity was found in the membrane preparation. This new type of acquired glycopeptide resistance was named VanE.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents / classification
  • Anti-Bacterial Agents / pharmacology*
  • Base Sequence
  • Drug Resistance, Microbial / genetics
  • Enterococcus faecalis / drug effects*
  • Enterococcus faecalis / enzymology
  • Enterococcus faecalis / genetics*
  • Genotype
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Peptidoglycan / biosynthesis
  • Phenotype
  • Racemases and Epimerases / metabolism
  • Teicoplanin / pharmacology*
  • Vancomycin / classification
  • Vancomycin / pharmacology*


  • Anti-Bacterial Agents
  • Peptidoglycan
  • Teicoplanin
  • Vancomycin
  • Racemases and Epimerases
  • serine racemase

Associated data

  • GENBANK/AF136925