Natural antioxidant, chlorogenic acid, protects against DNA breakage caused by monochloramine

Biosci Biotechnol Biochem. 1999 Jul;63(7):1295-7. doi: 10.1271/bbb.63.1295.

Abstract

Chlorogenic acid prevented a stepwise conversion of plasmid pUC18 DNA, from I-->form II-->form III, induced by 3 mM monochloramine with a half inhibition of 67.4 microM. Chlorogenic acid reacted with monochloramine in a time-dependent manner, and the reaction rate increased with decreasing pH. These results suggest that chlorogenic acid prevents genotoxicity of monochloramine in gastric mucosa.

MeSH terms

  • Ammonium Chloride / chemistry
  • Antioxidants / pharmacology*
  • Chloramines / antagonists & inhibitors*
  • Chloramines / toxicity*
  • Chlorogenic Acid / pharmacology*
  • DNA Damage / drug effects*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / drug effects
  • Helicobacter pylori / drug effects
  • Helicobacter pylori / metabolism
  • Plasmids / chemistry
  • Spectrophotometry, Ultraviolet

Substances

  • Antioxidants
  • Chloramines
  • DNA, Bacterial
  • Ammonium Chloride
  • Chlorogenic Acid
  • chloramine