Abstract
Chlorogenic acid prevented a stepwise conversion of plasmid pUC18 DNA, from I-->form II-->form III, induced by 3 mM monochloramine with a half inhibition of 67.4 microM. Chlorogenic acid reacted with monochloramine in a time-dependent manner, and the reaction rate increased with decreasing pH. These results suggest that chlorogenic acid prevents genotoxicity of monochloramine in gastric mucosa.
MeSH terms
-
Ammonium Chloride / chemistry
-
Antioxidants / pharmacology*
-
Chloramines / antagonists & inhibitors*
-
Chloramines / toxicity*
-
Chlorogenic Acid / pharmacology*
-
DNA Damage / drug effects*
-
DNA, Bacterial / chemistry
-
DNA, Bacterial / drug effects
-
Helicobacter pylori / drug effects
-
Helicobacter pylori / metabolism
-
Plasmids / chemistry
-
Spectrophotometry, Ultraviolet
Substances
-
Antioxidants
-
Chloramines
-
DNA, Bacterial
-
Ammonium Chloride
-
Chlorogenic Acid
-
chloramine