Aberrant wound healing and TGF-beta production in the autoimmune-prone MRL/+ mouse

Clin Immunol. 1999 Sep;92(3):300-10. doi: 10.1006/clim.1999.4754.

Abstract

Wound healing is a complex process that involves inflammation, apoptosis, growth, and tissue remodeling. The autoimmune-prone inbred mouse strain MRL/+ manifests accelerated and extensive healing to ear punch wounds, suggesting a link between immune defects and wound healing. Prior studies with lupus-prone mice have shown that hematopoietic cells of lupus-prone strains can transfer disease to otherwise non-autoimmune-prone recipients. In this study we performed reciprocal bone marrow transfers between MRL and the control strain B10.BR and found that radioresistant MRL/+ host cells, rather than hematopoietic cells, are required for the healing response. We have also made the novel observations that, compared to normal controls, MRL/+ hematopoietic cells overproduce TGF-beta1 and manifest impaired inflammatory responses to lipopolysaccharide challenge. These features suggest that the aberrant wound healing phenotype of MRL mice is independent of their propensity to develop autoimmunity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aging / genetics
  • Aging / physiology
  • Animals
  • Bone Marrow Transplantation
  • Bronchoalveolar Lavage Fluid / cytology
  • Genotype
  • Hematopoietic Stem Cells / metabolism
  • Inflammation Mediators / metabolism
  • Lipopolysaccharides / pharmacology
  • Mice
  • Mice, Inbred MRL lpr / metabolism*
  • Neutrophils / cytology
  • Pneumonia / physiopathology
  • Transforming Growth Factor beta / metabolism*
  • Transplantation Chimera
  • Wound Healing / genetics
  • Wound Healing / physiology*

Substances

  • Inflammation Mediators
  • Lipopolysaccharides
  • Transforming Growth Factor beta