Association between M/L55-polymorphism of paraoxonase enzyme and oxidative DNA damage in patients with type 2 diabetes mellitus and in control subjects

Hum Genet. Jul-Aug 1999;105(1-2):179-80. doi: 10.1007/s004399900074.

Abstract

The paraoxonase enzyme (PON) gene polymorphism causes a change of methionine (M-allele) to leucine (L-allele). PON may reduce low density lipoprotein oxidation and prevent atherosclerosis. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a sensitive index of oxidative DNA damage. We have studied the association between the PON genotypes and the urinary excretion of 8-OHdG. The study population consisted of 93 Finnish type 2 diabetes patients and 106 non-diabetic control subjects. The 24-h excretion of 8-OHdG was significantly higher in diabetic patients than in control subjects (P < 0.001). In control subjects, the ratio of the 8-OHdG/glomerular filtration rate increased in order of genotype from MM to ML to LL (P < 0.0412). These results suggest that lipid peroxidation may have an effect on DNA oxidation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Aryldialkylphosphatase
  • Case-Control Studies
  • DNA Damage*
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / urine
  • Diabetes Mellitus, Type 2 / genetics*
  • Esterases / genetics*
  • Female
  • Genotype
  • Humans
  • Lipid Peroxidation
  • Male
  • Polymorphism, Genetic

Substances

  • 8-Hydroxy-2'-Deoxyguanosine
  • Esterases
  • Aryldialkylphosphatase
  • Deoxyguanosine