Early Elicitor Induction in Members of a Novel Multigene Family Coding for Highly Related RING-H2 Proteins in Arabidopsis Thaliana

Plant Mol Biol. 1999 Jul;40(4):579-90. doi: 10.1023/a:1006267201855.


We describe the identification and structural characterization of a novel family of Arabidopsis genes related to ATL2 which encode a variant of the RING zinc finger domain, known as RING-H2. Analysis of genes selected by us and of sequences from Arabidopsis stored in databases permitted the prediction of several RING-H2 proteins that contain highly homologous RING domains. The ATL gene family is represented by fifteen sequences that contain, in addition to the RING, a transmembrane domain which is located in most of them towards the N-terminal end. Transgenic Arabidopsis seedlings carrying the ATL2 promoter fused to the GUS reporter gene revealed that the expression of ATL2 is rapidly induced after exposure to chitin or inactivated crude cellulase preparations. Rapid induction of transcript accumulation of another member of the ATL family was also observed under the same conditions. These results suggest that some ATLs may be involved in the early stages of the defense response triggered in plants in response to pathogen attack.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / chemistry
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Arabidopsis Proteins*
  • Cellulase / pharmacology
  • Chitin / pharmacology
  • Cycloheximide / pharmacology
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • DNA-Binding Proteins / genetics
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Plant
  • Glucuronidase / drug effects
  • Glucuronidase / genetics
  • Glucuronidase / metabolism
  • Molecular Sequence Data
  • Multigene Family / genetics*
  • Plants / drug effects
  • Plants / genetics
  • Promoter Regions, Genetic / genetics
  • Protein Synthesis Inhibitors / pharmacology
  • RNA, Messenger / drug effects
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Recombinant Fusion Proteins / drug effects
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Zinc Fingers / genetics*


  • ATL2 protein, Arabidopsis
  • Arabidopsis Proteins
  • DNA, Complementary
  • DNA-Binding Proteins
  • Protein Synthesis Inhibitors
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Chitin
  • Cycloheximide
  • Glucuronidase
  • Cellulase

Associated data

  • GENBANK/AF132013
  • GENBANK/AF132014
  • GENBANK/AF132015
  • GENBANK/AF132016