IgE reactivity to alpha1 and alpha2 chains of bovine type 1 collagen in children with bovine gelatin allergy

J Allergy Clin Immunol. 1999 Sep;104(3 Pt 1):695-9. doi: 10.1016/s0091-6749(99)70344-1.


Background: Anaphylactic reactions to measles, mumps, and rubella vaccines, including gelatin as a stabilizer, have been reported. It had been found that most of these reactions to live vaccines are caused by the bovine gelatin included in these vaccines. Gelatin mainly includes denatured type I collagen, which consists of alpha1 and alpha2 chains.

Objective: The current study was designed to investigate the IgE reactivity to alpha1 and alpha2 chains of bovine type I collagen in gelatin-sensitive children.

Methods: Serum samples were taken from 10 children who had anaphylaxis to the vaccines and high levels of specific IgE to bovine gelatin. Bovine type I collagen was isolated from bovine skin and then separated to alpha1 and alpha2 chains by column chromatography. IgE reactivity to denatured type I collagen and its alpha1 and alpha2 chains was analyzed by immunoblotting, ELISA, and histamine release from the mast cells passive sensitized with IgE antibodies in pooled serum of the children.

Results: All children had specific IgE to bovine type I collagen. Furthermore, IgE antibodies in their sera reacted with the alpha;2 chain but not with the alpha1 chain. Similarly, the mast cells sensitized with pooled sera in the children showed alpha2 chain-specific histamine release but not alpha1 chain-specific histamine release.

Conclusion: In gelatin allergy denatured bovine type I collagen is a major allergen and IgE-binding sites exist in the alpha2 chain of type I collagen.

Publication types

  • Clinical Trial

MeSH terms

  • Anaphylaxis / immunology*
  • Animals
  • Cattle
  • Cells, Cultured
  • Child, Preschool
  • Collagen / immunology*
  • Excipients
  • Female
  • Gelatin / immunology*
  • Histamine Release
  • Humans
  • Immunoglobulin E / immunology*
  • Infant
  • Male
  • Mast Cells / cytology
  • Mast Cells / immunology
  • Protein Denaturation
  • Vaccines / immunology


  • Excipients
  • Vaccines
  • Immunoglobulin E
  • Gelatin
  • Collagen