Electrically silent potassium channel subunits from human lens epithelium

Am J Physiol. 1999 Sep;277(3):C412-24. doi: 10.1152/ajpcell.1999.277.3.C412.


We describe the cloning and characterization of the first human members, hKv9.1 and hKv9.3, of the electrically silent delayed-rectifying-like K+ channel subfamily. Their modulatory effects on the electrically active subfamily member hKv2.1 are also quantified. The hKv9 K+ channels were isolated from a human lens epithelium cDNA library, but both hKv9.1 mRNA and hKv9.3 mRNA were found to coexist with the mRNA for hKv2.1 in a large number of human tissues. The hKv9.1 gene is composed of a minimum of five exons, with at least two alternatively spliced exons in the 5'-untranslated region (UTR). In contrast, the hKv9.3 gene is intronless across the coding region, 3'-UTR, and all of the analyzed 5'-UTR. Radiation hybrid mapping localized the hKv9.1 gene to 20q12 and the hKv9.3 gene to 2p24. Each electrically silent subunit, when coexpressed with hKv2.l, slows deactivation and inactivation compared with hKv2. 1 expressed alone. In addition, each results in an increment in the single channel conductance.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence / genetics
  • Animals
  • CHO Cells
  • COS Cells
  • Cell Line
  • Chromosome Mapping
  • Cricetinae
  • Delayed Rectifier Potassium Channels
  • Electrophysiology
  • Epithelium / metabolism
  • Humans
  • Lens, Crystalline / metabolism*
  • Molecular Sequence Data
  • Potassium Channels / genetics
  • Potassium Channels / metabolism
  • Potassium Channels / physiology*
  • Potassium Channels, Voltage-Gated*
  • Tissue Distribution
  • Transfection


  • Delayed Rectifier Potassium Channels
  • KCNS1 protein, human
  • Potassium Channels
  • Potassium Channels, Voltage-Gated

Associated data

  • GENBANK/AF043472
  • GENBANK/AF043473