Stimulation of Lipolysis but Not of Leptin Release by Growth Hormone Is Abolished in Adipose Tissue From Stat5a and B Knockout Mice

Biochem Biophys Res Commun. 1999 Sep 16;263(1):201-5. doi: 10.1006/bbrc.1999.1302.

Abstract

The present studies examined the effects of growth hormone (GH) on lipolysis and leptin release by adipose tissue from mice incubated for 24 h in primary culture. In adipose tissue from control mice GH enhanced lipolysis without affecting leptin release. The lipolytic action of GH was unaffected in adipose tissue from Stat5b-/- male mice but leptin release was enhanced by GH in fat from Stat5b-/- mice. In adipose tissue from Stat5ab-/- female mice no significant lipolytic action of GH was seen but leptin release was enhanced by GH. An insulin-like effect of GH on glucose conversion to lactate was also seen in mice deficient in Stat5ab-/-. These results suggest that the lipolytic action of GH involves the Stat5 proteins while the insulin-like effects of GH on glucose metabolism and leptin release involve different mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adipose Tissue / drug effects*
  • Adipose Tissue / physiology*
  • Animals
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology*
  • Female
  • Glucose / metabolism
  • Human Growth Hormone / pharmacology*
  • Humans
  • Leptin
  • Lipolysis / drug effects*
  • Male
  • Mice
  • Mice, Knockout
  • Milk Proteins*
  • Proteins / metabolism*
  • Receptors, Glucocorticoid / physiology
  • STAT5 Transcription Factor
  • Signal Transduction
  • Trans-Activators / genetics
  • Trans-Activators / physiology*
  • Tumor Suppressor Proteins

Substances

  • DNA-Binding Proteins
  • Leptin
  • Milk Proteins
  • Proteins
  • Receptors, Glucocorticoid
  • STAT5 Transcription Factor
  • STAT5A protein, human
  • STAT5B protein, human
  • Stat5a protein, mouse
  • Stat5b protein, mouse
  • Trans-Activators
  • Tumor Suppressor Proteins
  • Human Growth Hormone
  • Glucose