The role of chloroplastic NAD(P)H dehydrogenase in photoprotection

FEBS Lett. 1999 Aug 20;457(1):5-8. doi: 10.1016/s0014-5793(99)00989-8.

Abstract

After a brief exposure to supra-saturating light, leaves of a tobacco transformant, in which chloroplastic NAD(P)H dehydrogenase (NDH) was defective, showed more severe photoinhibition than the wild-type, when judged by the parameter of chlorophyll fluorescence Fv/Fm. Repeated application of supra-saturating light eventually resulted in chlorosis in the NDH-defective mutant, while the wild-type sustained less photodamage and was able to recover from it. The mechanism of the phenomena is discussed with respect to the potential role of NDH in photosynthesis.

MeSH terms

  • Chloroplasts / enzymology*
  • Ferredoxin-NADP Reductase / metabolism
  • Light / adverse effects*
  • Light-Harvesting Protein Complexes
  • Mutagenesis, Insertional
  • NADPH Dehydrogenase / physiology*
  • Oxidation-Reduction
  • Photosynthesis / physiology
  • Photosynthetic Reaction Center Complex Proteins / metabolism
  • Plant Proteins / physiology
  • Plants, Toxic
  • Time Factors
  • Tobacco / enzymology
  • Tobacco / radiation effects
  • Transformation, Genetic

Substances

  • Light-Harvesting Protein Complexes
  • Photosynthetic Reaction Center Complex Proteins
  • Plant Proteins
  • Ferredoxin-NADP Reductase
  • NADPH Dehydrogenase