Low temperature and aging-promoted expression of PUMP in potato tuber mitochondria

FEBS Lett. 1999 Aug 20;457(1):103-6. doi: 10.1016/s0014-5793(99)01017-0.


In this communication, we show that the plant uncoupling mitochondrial protein (PUMP) present in potato tuber mitochondria is induced by aging at 28 degrees C and that this induction is strongly stimulated when the potato tubers are stored at low temperature (4 degrees C). PUMP activity was detected by the degree of linoleic acid (LA)-induced ATP-sensitive mitochondrial uncoupling measured as a function of the decrease in membrane potential (delta psi). The PUMP content was evaluated by immunoblot analysis using polyclonal antibodies raised against potato PUMP that specifically detected a 32 kDa band. In agreement with the effect of LA on delta psi, the content of the 32 kDa band increased during storage and was stimulated by low temperature. These results support the proposed role of PUMP in plant thermogenesis and possibly in fruit ripening and senescence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carrier Proteins / metabolism*
  • Immunoblotting
  • Ion Channels
  • Membrane Potentials
  • Membrane Proteins / metabolism*
  • Mitochondria / metabolism*
  • Mitochondrial Proteins
  • Plant Proteins / metabolism*
  • Solanum tuberosum / metabolism*
  • Temperature*
  • Time Factors
  • Uncoupling Protein 1


  • Carrier Proteins
  • Ion Channels
  • Membrane Proteins
  • Mitochondrial Proteins
  • Plant Proteins
  • Uncoupling Protein 1