Development of a monoclonal antibody blocking-ELISA for detection of antibodies against Maedi-Visna virus

J Virol Methods. 1999 Aug;81(1-2):101-8. doi: 10.1016/s0166-0934(99)00061-0.


A monoclonal antibody (MAb) blocking ELISA (Blck-ELISA) was developed to detect antibodies against Maedi-Visna virus (MVV) in sheep sera. The assay employs a MAb directed against the envelope protein p90 of the virus in a sandwich blocking procedure. To determine whether the MAb was a potential antibody for developing a Blck-ELISA, a collection of three hundred sera obtained from several sheep flocks known to be infected with MVV were used to examine the sensitivity of the Blck-ELISA. A total of 50 serum samples originating from a flock free of MVV were tested to assess the specificity of the assay. The results were compared with a commercial indirect ELISA (I-ELISA) and samples giving a conflicting or doubtful result were tested by immunoblot. The Blck-ELISA proved to be specific, sensitive and it showed high reproducibility and low variability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Blocking / biosynthesis*
  • Antibodies, Blocking / chemistry
  • Antibodies, Blocking / pharmacology*
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / pharmacology*
  • Antibodies, Viral / analysis*
  • Cells, Cultured
  • Choroid Plexus
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Enzyme-Linked Immunosorbent Assay / standards
  • Mice
  • Mice, Inbred BALB C
  • Sensitivity and Specificity
  • Sheep
  • Viral Envelope Proteins / immunology
  • Visna-maedi virus / immunology*


  • Antibodies, Blocking
  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Viral Envelope Proteins