Rapid Method for the Identification of Essential Genes in Staphylococcus Aureus

Plasmid. 1999 Sep;42(2):144-9. doi: 10.1006/plas.1999.1422.


A strategy based on a vector host-dependent for autonomous replication, pSA3182, was utilized both for the rapid screening for Staphylococcus aureus genes essential for cell viability and for the introduction of specific polarity-neutral deletions in nonessential genes. The results obtained support the use of pSA3182 for both purposes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • CCAAT-Enhancer-Binding Proteins*
  • DEAD-box RNA Helicases
  • DNA-Binding Proteins / genetics
  • Escherichia coli Proteins*
  • Genes, Bacterial*
  • Genes, Essential*
  • NFI Transcription Factors
  • Peptide Elongation Factors / genetics
  • RNA Helicases / genetics
  • RNA-Binding Proteins*
  • Staphylococcus aureus / genetics*
  • Time Factors
  • Transcription Factors / genetics


  • Bacterial Proteins
  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • NFI Transcription Factors
  • NusG protein, E coli
  • Peptide Elongation Factors
  • RNA-Binding Proteins
  • Transcription Factors
  • dbpA protein, E coli
  • DEAD-box RNA Helicases
  • RNA Helicases