Abstract
Activated Th1 CD4 T cells bind to P-selectin and migrate into inflamed tissue, whereas Th2 cells do not. We show that alpha(1, 3)-fucosyltransferase VII (FucT-VII) and alpha(2, 3)-sialyltransferase IV (ST3GalIV), which are crucial for the biosynthesis of functional P-selectin ligands, are absent in naive CD4 T cells, but are rapidly up-regulated upon activation. Th1 or Th2 differentiation in the presence of polarizing cytokines leads to down-regulation of FucT-VII mRNA selectively in Th2 but not in Th1 cells. Influencing the differentiation by varying the priming dose of antigenic peptide results in similar FucT-VII down-regulation only in Ag-specific Th2 cells. ST3GalIV levels remain elevated. FucT-VII and ST3GalIV mRNAs are also up-regulated by Th1 cells primed in vivo and recruited into the lymph nodes draining delayed-type hypersensitivity sites. We identify FucT-VII gene expression as a principal difference between Th1 and Th2 cells, and underscore the importance of FucT-VII and ST3GalIV expression for the biosynthesis of functional selectin ligands.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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CD4-Positive T-Lymphocytes / enzymology*
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CD4-Positive T-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / pathology
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Cell Differentiation / immunology
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Cell Movement / immunology*
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Down-Regulation / immunology
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Epitopes, T-Lymphocyte / biosynthesis
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Fucosyltransferases / biosynthesis*
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Fucosyltransferases / genetics
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Gangliosides / immunology
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Histocompatibility Antigens Class II / metabolism
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Hypersensitivity, Delayed / enzymology
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Hypersensitivity, Delayed / immunology
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Hypersensitivity, Delayed / pathology
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Interleukin-12 / pharmacology
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Interleukin-4 / pharmacology
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Interphase / immunology
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Lewis Blood Group Antigens / immunology
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Lymph Nodes / enzymology
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Lymph Nodes / immunology
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Lymph Nodes / pathology*
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Lymphocyte Activation*
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C3H
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Mice, Inbred C57BL
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Mice, Transgenic
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Peptide Fragments / immunology
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Peptide Fragments / metabolism
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RNA, Messenger / biosynthesis
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Sialyl Lewis X Antigen
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Sialyltransferases / biosynthesis*
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Th1 Cells / enzymology*
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Th1 Cells / immunology
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Th1 Cells / pathology
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Th2 Cells / enzymology
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Th2 Cells / immunology
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Up-Regulation / immunology
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beta-Galactoside alpha-2,3-Sialyltransferase
Substances
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Epitopes, T-Lymphocyte
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Gangliosides
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Histocompatibility Antigens Class II
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Lewis Blood Group Antigens
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Peptide Fragments
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RNA, Messenger
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Sialyl Lewis X Antigen
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sialyl Lewis(x) ganglioside
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Interleukin-12
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Interleukin-4
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Fucosyltransferases
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galactoside 3-fucosyltransferase
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Sialyltransferases
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beta-Galactoside alpha-2,3-Sialyltransferase