Suicide gene-mediated modulation of graft-versus-host disease

Leuk Lymphoma. 1999 Aug;34(5-6):473-80. doi: 10.3109/10428199909058474.


The development of suicide genes and progress in retroviral gene transfer to T-cells open new perspectives for the treatment of graft-versus-host disease (GVHD) after allogeneic bone marrow transplantation (BMT) for leukemia and lymphoma. Indeed, suicide genes that metabolize inactive prodrugs into compounds toxic for dividing cells provide a powerful means for the pharmacogenetic control of T-cell reactivity. Here, we demonstrate the selective destruction of activated TK-transgenic T-cells in vivo and develop two new transgenic lines which should be useful for preclinical studies of suicide gene therapy strategies for GVHD.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Apoptosis
  • Bone Marrow Transplantation
  • Ganciclovir / pharmacology
  • Genetic Therapy*
  • Graft vs Host Disease / genetics*
  • Graft vs Host Disease / pathology
  • Graft vs Host Disease / therapy*
  • Hematologic Neoplasms / therapy
  • Humans
  • Lymphocyte Depletion / methods
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • T-Lymphocytes / cytology
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / transplantation
  • Thymidine Kinase / genetics
  • Thymidine Kinase / metabolism


  • Thymidine Kinase
  • Ganciclovir