Evaluation of the direct agglutination test (DAT) using freeze-dried antigen for the detection of anti-Leishmania antibodies in stored sera from various patient groups in Ethiopia

Trans R Soc Trop Med Hyg. May-Jun 1999;93(3):275-7. doi: 10.1016/s0035-9203(99)90021-4.

Abstract

Tests on a panel of 421 serum samples from Ethiopia and 11 from Europe with the direct agglutination test (DAT) and freeze-dried antigen (Leishmania donovani 1S) showed that a cut-off titre of 1:200 or 1:400 could be employed. Using a cut-off titre of 1:400, 181 (88.3%) of 205 serum samples from visceral leishmaniasis (VL) patients tested positive, as did 5 (13.9%) of 36 sera from patients with localized cutaneous leishmaniasis, 5 (71.4%) sera from 7 patients with diffuse cutaneous leishmaniasis and 8 (89.9%) sera from 9 HIV-positive VL patients. We also found that 19 (57.6%) sera from 33 treated VL patients were still positive in the DAT at 1.5 to 5 years after treatment.

MeSH terms

  • Agglutination Tests*
  • Antibodies, Protozoan / analysis*
  • Blood Preservation
  • Ethiopia
  • Freeze Drying
  • Humans
  • Leishmaniasis / diagnosis*
  • Leishmaniasis, Cutaneous / diagnosis
  • Leishmaniasis, Cutaneous / therapy
  • Leishmaniasis, Visceral / diagnosis
  • Leishmaniasis, Visceral / therapy
  • Sensitivity and Specificity
  • Time Factors

Substances

  • Antibodies, Protozoan