Ly-49 receptor expression and functional analysis in multiple mouse strains

J Leukoc Biol. 1999 Sep;66(3):512-20. doi: 10.1002/jlb.66.3.512.


We present data on the strain distribution and functional characteristics of the Ly-49 receptors A, C/I, D, and G2 on DX5+ natural killer (NK) cells. We have examined tyrosine phosphorylation of the Ly-49 molecules, regulation of NK cytotoxic functions, and in vivo marrow rejection capability. The flow cytometry results demonstrate a diverse and complex pattern of expression of the Ly-49 receptors in the 11 strains examined. The vast majority of NK cells express Ly-49s, although some NK1.1+ CD3+ cells also express these receptors. The results of our functional analysis indicate that H-2Dd was able to inhibit the function of Ly-49G2+ NK cells, not only in B6 mice, but also by NK cells derived from several haplotypes. The examination of Ly-49 receptor tyrosine phosphorylation, which is a biochemical measure of inhibitory function, was consistently observed in the 11 mouse strains examined. In contrast, analysis of Ly-49D function suggests its expression appears to be more restricted and that H-2Dd is an activating ligand for this receptor. In addition, the in vivo examination of both inhibitory (Ly-49G2) and activating (Ly-49D) receptors demonstrated regulatory roles of these class I binding receptors in marrow transplantation.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Ly*
  • Bone Marrow Transplantation / immunology
  • Cytotoxicity, Immunologic
  • Graft Rejection / immunology
  • H-2 Antigens / immunology
  • Haplotypes / genetics
  • Histocompatibility Antigen H-2D
  • Killer Cells, Natural / immunology
  • Killer Cells, Natural / metabolism*
  • Lectins, C-Type
  • Ligands
  • Liver / cytology
  • Liver / immunology
  • Membrane Glycoproteins / biosynthesis
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / immunology
  • Membrane Glycoproteins / physiology*
  • Mice
  • Mice, Inbred Strains / genetics
  • Mice, Inbred Strains / immunology*
  • Mice, Nude
  • Phosphorylation
  • Phosphotyrosine / analysis
  • Protein Isoforms / biosynthesis
  • Protein Isoforms / genetics
  • Protein Isoforms / immunology
  • Protein Isoforms / physiology
  • Protein Processing, Post-Translational
  • Radiation Chimera
  • Receptors, NK Cell Lectin-Like
  • Species Specificity
  • Specific Pathogen-Free Organisms
  • Tumor Cells, Cultured


  • Antigens, Ly
  • H-2 Antigens
  • Histocompatibility Antigen H-2D
  • Lectins, C-Type
  • Ligands
  • Membrane Glycoproteins
  • Protein Isoforms
  • Receptors, NK Cell Lectin-Like
  • Phosphotyrosine