Effects of single and repeated stresses on the expression of mRNA for alpha1-adrenoceptors in the rat hypothalamus and midbrain

Eur J Pharmacol. 1999 Aug 20;379(1):111-4. doi: 10.1016/s0014-2999(99)00498-7.

Abstract

We examined the effects of single or repeated stress on the expression of mRNA for alpha1-adrenoceptors in the rat hypothalamus and midbrain using the reverse transcriptase-polymerase chain reaction (RT-PCR). Single stress significantly increased the mRNA level for alpha1-adrenoceptors in the midbrain, but had no effect on mRNA levels in the hypothalamus. Repeated stress significantly decreased mRNA levels for alpha1-adrenoceptors in both regions.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Hypothalamus / metabolism*
  • Male
  • Mesencephalon / metabolism*
  • RNA, Messenger / genetics*
  • Rats
  • Rats, Wistar
  • Receptors, Adrenergic, alpha-1 / genetics*
  • Restraint, Physical / adverse effects*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stress, Mechanical
  • Time Factors

Substances

  • RNA, Messenger
  • Receptors, Adrenergic, alpha-1