Identification of a Drosophila brain-gut peptide related to the neuropeptide Y family

Peptides. 1999;20(9):1035-42. doi: 10.1016/s0196-9781(99)00097-2.


A neuropeptide F (NPF) was isolated from the fruit fly, Drosophila mellanogaster, based on a radioimmunoassay for a gut peptide from the corn earworm, Helicoverpa zea. A partial sequence was obtained from the fly peptide, and a genomic sequence coding for NPF was cloned after inverse polymerase chain reaction and shown to exist as a single genomic copy. The encoded, putative prepropeptide can be processed into an amidated NPF with 36 residues that is related to invertebrate NPF's and the neuropeptide Y family of vertebrates. In situ hybridization and immunocytochemistry showed that Drosophila NPF was expressed in the brain and midgut of fly larvae and adults.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain Chemistry
  • Cloning, Molecular
  • DNA
  • Drosophila Proteins
  • Drosophila melanogaster / chemistry*
  • Immunohistochemistry
  • In Situ Hybridization
  • Intestines / chemistry
  • Mass Spectrometry
  • Molecular Sequence Data
  • Neuropeptide Y / chemistry*
  • Neuropeptides / chemistry*
  • Neuropeptides / genetics
  • Polymerase Chain Reaction
  • Sequence Homology, Amino Acid


  • Drosophila Proteins
  • Neuropeptide Y
  • Neuropeptides
  • neuropeptide F, Drosophila
  • DNA